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Cat. Number
E-006
Chemical Name
新型冠状病毒(COVID-19)RBD蛋白 (2019-nCoV)Spike Protein(RBD,mFc Tag)
CAS Number
E006
Qty 1
1mg
Qty 2
100mg
Application Notes
> 95 % as determined by SDS-PAGE.
Synonym
RBD蛋白mFc Tag;cov spike Protein, 2019-nCoV;coronavirus spike Protein, 2019-nCoV
Storage condition
-20℃ to -80℃
References

2019-nCoV Spike Protein (RBD, Fc Tag)
新型冠状病毒RBD蛋白产品信息

纯度:> 95 % as determined by SDS-PAGE.

蛋白质序列:RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFADDDDKAVPRDSGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

内毒素< 1.0 EU per μg protein as determined by the LAL method.

生物活性

    Measured by its binding ability in a functional ELISA.
Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL.

蛋白构建

    A DNA sequence encoding the NCP-CoV(2019-nCoV) Spike Protein (RBD) was expressed with the Fc region of mouse IgG1 at the C-terminus.

表达宿主:HEK293 Cells

种属:2019-nCoV

预测 N 端:Arg

分子量The recombinant NCP-CoV(2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.

缓冲液    Lyophilized from sterile PBS, pH 7.4.

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to the specific buffer information in the hard copy of CoA.

运输方式

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

  &nbsp; A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

研究领域

  • Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen

参考文献

  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.

  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.

  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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上海惠诚生物GMP级细胞培养产品列表:

货号名称规格
GMP-001重组抗PD-1全人单克隆抗体1.0mg
GMP-002重组Her-2全人单克隆抗体2mg/10mg
GMP-003鼠抗人CD3单克隆抗体0.5mg/1mg
GMP-004鼠抗人CD28单克隆抗体0.5mg/1mg
GMP-005重组人Activin A50ug/1.0mg
GMP-006重组人脑源性神经营养因子(rhBDNF)50ug/1.0mg
GMP-007重组人碱性成纤维细胞生长因子(rhbFGF)50ug/1.0mg
GMP-008重组人BMP-2(rhBMP-2)50ug/1.0mg
GMP-009重组人BMP-4(rhBMP-4)50ug/1.0mg
GMP-010重组人BMP-7(rhBMP-7)50ug/1.0mg
GMP-011重组人CD40L(rhCD40L)50ug/1.0mg
GMP-012重组人Dkk1(rhDkk1)50ug/1.0mg
GMP-013重组人表皮生长因子(rhEGF)1.0mg
GMP-014重组人促红细胞生成素(rhEPO)1.0mg
GMP-015重组人Flt3L(rhFLt3L)50ug/1.0mg
GMP-016重组人角质细胞生长因子-2(rhKGF-2&FGF-10)50ug/1.0mg
GMP-017重组人成纤维细胞生长因子-4(rhFGF4)50ug/1.0mg
GMP-018重组人成纤维细胞生长因子-9(rhFGF9)50ug/1.0mg
GMP-019重组人纤维连接蛋白(rhFN)1mg
GMP-020重组人胶质细胞源性神经营养因子(rhGDNF)50ug/1.0mg
GMP-021重组人肝细胞生长因子(rhHGF)50ug/1.0mg
GMP-022重组人干扰素-γ(rhIFN-γ)100ug/1.0mg
GMP-023重组人白细胞介素-1a(rhIL-1a)50ug/1.0mg
GMP-024重组人白细胞介素-2(rhIL-2)100ug/1.0mg
GMP-025重组人白细胞介素-3(rhIL-3)50ug/1.0mg
GMP-026重组人白细胞介素-4(rhIL-4)50ug/1.0mg
GMP-027重组人白细胞介素-6(rhIL-6)50ug/1.0mg
GMP-028重组人白细胞介素-7(rhIL-7)50ug/1.0mg
GMP-029重组人白细胞介素-12(rhIL-12)50ug/1.0mg
GMP-030重组人白细胞介素-15(rhIL-15)50ug/1.0mg
GMP-031重组人白细胞介素-18(rhIL-18)50ug/1.0mg
GMP-032重组人白细胞介素-21(rhIL-21)50ug/1.0mg
GMP-033重组人白血病抑制因子(rhLIF)50ug/1.0mg
GMP-034重组人长链胰岛素样生长因子-1(rhLR3-IGF-1)1.0mg
GMP-035重组人Noggin(rhNoggin)50ug/1.0mg
GMP-036重组人NRG-1(rhNRG-1)50ug/1.0mg
GMP-037重组人血小板源生长因子(rhPDGF-BB)1.0mg
GMP-038重组人R-spondin(rhR-spondin)50ug/1.0mg
GMP-039重组人干细胞因子(rhSCF)50ug/1.0mg
GMP-040重组人音猬因子(rhSHH)50ug/1.0mg
GMP-041重组人肿瘤坏死因子(rhTNF-a)50ug/1.0mg
GMP-042重组人转化生长因子(rhTGFβ1)50ug/1.0mg
GMP-043重组人血管内皮生长因子(rhVEGF)50ug/1.0mg
GMP-044重组人Wnt-3α(rhWnt-3α)50ug/1.0mg
GMP-045重组人胰岛素(rhInsulin)1.0g/10.0g
GMP-046牛转铁蛋白(Bovine Transferrin) inactivated virus1.0g/10.0g
GMP-047重组人干扰素α2b1.0mg
GMP-048重组人粒细胞集落刺激因子1.0mg
E-001重组CRISPR-Cas9蛋白100/500pmol
E-002重组CRISPR-Cas12a蛋白100/2000pmol
E-003重组CRISPR-Cas13a蛋白100/500pmol
E-004重组SUMO蛋白酶1000U
E-005重组TEV蛋白酶1000/10000U
E-006Spike Protein (RBD, mouseFc Tag)1mg/10mg
E-007Spike Protein (S1 Subunit, His Tag)1mg/10mg
VISC2-S002重组全长S蛋白 100ug/1.0mg
VISC2-RB04RBD蛋白 (RBD Subunit,His Tag)100ug/1.0mg
VISC2-RB05RBD-591蛋白(RBD-591 Subunit,His Tag)100ug/1.0mg


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